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A three-enzyme microelectrode sensor for detecting purine release from central nervous system.

Enrique Llaudet·Nigel P Botting·Joe A Crayston·Nicholas Dale
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Abstract

As the purines, in particular adenosine, are important signaling agents in the nervous system we have devised a new biosensor for directly measuring their production in real time during physiological activity. Our amperometric adenosine biosensor is made by entrapping 3 enzymes (xanthine oxidase, purine nucleoside phosphorylase and adenosine deaminase) in a composite lactobionamide and amphiphillic polypyrrole matrix around a Pt microelectrode. The resulting sensors are small (25-100 microm diameter), fast responding (10-90% rise time, 2+/-0.23 s), sensitive (100-222 mA M(-1) cm(-2)) and stable (100% activity after 5 days). The sensor was used in vivo to demonstrate the spatial localization of release of adenosine from Xenopus embryo spinal cord during fictive swimming.

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AnimalsBiosensing TechniquesCentral Nervous SystemElectrodesPurinesXenopusJournal ArticleResearch Support, Non-U.S. Gov't

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Enrique Llaudet, Nigel P Botting, Joe A Crayston, Nicholas Dale. 2003. A three-enzyme microelectrode sensor for detecting purine release from central nervous system.. https://doi.org/10.1016/s0956-5663(02)00106-9

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