Post-analysis follow-up and validation of microarray experiments.

Rodrigo F Chuaqui; Robert F Bonner; Carolyn J M Best; John W Gillespie; Michael J Flaig; Stephen M Hewitt; John L Phillips; David B Krizman; Michael A Tangrea; Mamoun Ahram; W Marston Linehan; Vladimir Knezevic; Michael R Emmert-Buck

doi:10.1038/ng1034

PubMed · 12454646

Post-analysis follow-up and validation of microarray experiments.

Rodrigo F Chuaqui·Robert F Bonner·Carolyn J M Best·John W Gillespie·Michael J Flaig·Stephen M Hewitt·John L Phillips·David B Krizman·Michael A Tangrea·Mamoun Ahram·W Marston Linehan·Vladimir Knezevic·Michael R Emmert-Buck

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Abstract

Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.

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Animals DNA, Complementary Forecasting Gene Expression Profiling Humans Oligonucleotide Array Sequence Analysis Proteins Quality Control RNA, Messenger Reproducibility of Results Sensitivity and Specificity Journal Article Research Support, U.S. Gov't, P.H.S.Review

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Rodrigo F Chuaqui, Robert F Bonner, Carolyn J M Best, John W Gillespie, Michael J Flaig, Stephen M Hewitt, John L Phillips, David B Krizman, Michael A Tangrea, Mamoun Ahram, W Marston Linehan, Vladimir Knezevic, Michael R Emmert-Buck. 2002. Post-analysis follow-up and validation of microarray experiments.. https://doi.org/10.1038/ng1034

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