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Pyrosequencing for microbial typing.

Mostafa Ronaghi·Elahe Elahi
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Abstract

Pyrosequencing is a real-time DNA sequencing technique generating short reads rapidly and inexpensively. This technology has the potential advantage of accuracy, ease-of-use, high flexibility and is now emerging as a popular platform for microbial typing. Here, we review the methodology and the use of this technique for viral typing, bacterial typing, and fungal typing. In addition, we describe how to use multiplexing for accurate and rapid typing.

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BacteriaSequence Analysis, DNAVirusesJournal ArticleResearch Support, U.S. Gov't, P.H.S.Review

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Mostafa Ronaghi, Elahe Elahi. 2002-12-25. Pyrosequencing for microbial typing.. https://doi.org/10.1016/s1570-0232(02)00693-1

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