Two efficient polymeric chemical platforms for oligonucleotide microarray preparation.

Clarissa Consolandi; Bianca Castiglioni; Roberta Bordoni; Elena Busti; Cristina Battaglia; Luigi Rossi Bernardi; Gianluca De Bellis

doi:10.1081/ncn-120015069

PubMed · 12484451

Two efficient polymeric chemical platforms for oligonucleotide microarray preparation.

Clarissa Consolandi·Bianca Castiglioni·Roberta Bordoni·Elena Busti·Cristina Battaglia·Luigi Rossi Bernardi·Gianluca De Bellis

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Abstract

In this report we describe two robust procedures for oligonucleotide microarray preparation based on polymeric coatings. The proposed chemical approaches include: 1) a glass functionalisation step with appropriate silanes (gamma-aminopropyltriethoxysilane-APTES or 3-glycidoxypropyltrimethoxysilane-GOPS), 2) a coating step using polymers (poly-L-Lysine or poly(acrylic acid-co-acrylamide) copolymer) covalently bound to the modified glass and 3) a surface activation step to allow for the attachment of amino-modified oligonucleotides. Results obtained using these chemistries in oligo microarray preparation show: 1) an overall high loading capacity and availability to hybridisation against targets, 2) a good uniformity, 3) resistance to consecutive probing/ stripping cycles, 4) stability to thermal cycles, 5) effectiveness in hybridisation-mediated mutation detection procedures and 6) the possibility to perform enzymatic reactions, such as ligation.

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Acrylamides Carbohydrate Conformation Carbohydrate Sequence DNA Mutational Analysis Genes, BRCA1 Glass Humans Molecular Structure Mutation Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Polymers Propylamines Silanes Comparative Study Journal Article Research Support, Non-U.S. Gov't

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Clarissa Consolandi, Bianca Castiglioni, Roberta Bordoni, Elena Busti, Cristina Battaglia, Luigi Rossi Bernardi, Gianluca De Bellis. 2002. Two efficient polymeric chemical platforms for oligonucleotide microarray preparation.. https://doi.org/10.1081/ncn-120015069

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