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Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes.

Ivanildo Luiz de Mattos·Lo Gorton·Tautgirdas Ruzgas
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Abstract

Gold (Au) and platinum (Pt) screen-printed electrodes were modified with Prussian Blue (PB) for the development of amperometric sensors selective for hydrogen peroxide detection. The sensors exhibited sensitivities towards H(2)O(2) equal to 2 A M(-1) cm(-2) for Au and 1 A M(-1) cm(-2) for Pt electrodes. The sensors were also employed as the basis for construction of glucose biosensors through further modification with crystallised glucose oxidase immobilised in a Nafion membrane. In order to improve the operational stability of the modified electrodes a buffer solution containing tetrabutylammonium toluene-4-sulfonate was used. The long-term performance of the sensors and biosensors were evaluated by continuous monitoring of hydrogen peroxide and glucose solutions (50 microM and 1 mM, respectively) in the flow-injection mode for 10 h.

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Biosensing TechniquesCoated Materials, BiocompatibleElectrochemistryElectrodesEnzymes, ImmobilizedEquipment DesignEquipment Failure AnalysisFerrocyanidesFlow Injection AnalysisGlucoseGlucose OxidaseGoldHydrogen PeroxideHydrogen-Ion ConcentrationLeadMaterials TestingPrussian Blue ReactionQuality ControlReproducibility of ResultsSensitivity and SpecificityComparative StudyEvaluation StudyJournal ArticleValidation Study

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Ivanildo Luiz de Mattos, Lo Gorton, Tautgirdas Ruzgas. 2003. Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes.. https://doi.org/10.1016/s0956-5663(02)00185-9

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