Live cell imaging using wide-field microscopy and deconvolution.

Jason R Swedlow; Melpomeni Platani

doi:10.1247/csf.27.335

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Live cell imaging using wide-field microscopy and deconvolution.

Jason R Swedlow·Melpomeni Platani

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Abstract

The use of fluorescence imaging methods, most recently based on fluorescent protein technology, and the availability of high quality fluorescence imaging systems have driven a revolution in cell and molecular biology. Live cell imaging, especially using fluorescence, is now used in a wide variety of assays in academic and commercial laboratories. The use of this technology requires particular attention to be paid to cell engineering, the design of the image acquisition system, the imaging protocol, and subsequent processing and analytic methods. In this review, we discuss each of these steps, highlighting practical techniques developed by us and others.

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Algorithms Animals Cell Culture Techniques Diffusion Chambers, Culture Eukaryotic Cells Fluorescent Dyes Green Fluorescent Proteins Humans Image Processing, Computer-Assisted Luminescent Proteins Microscopy, Fluorescence Signal Processing, Computer-Assisted Journal Article Research Support, Non-U.S. Gov't Review

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Jason R Swedlow, Melpomeni Platani. 2002. Live cell imaging using wide-field microscopy and deconvolution.. https://doi.org/10.1247/csf.27.335

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