Express primer tool for high-throughput gene cloning and expression.

J R Yoon; P D Laible; M Gu; H N Scott; F R Collart

doi:10.2144/02336bc03

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Express primer tool for high-throughput gene cloning and expression.

J R Yoon·P D Laible·M Gu·H N Scott·F R Collart

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Abstract

High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.

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Algorithms Base Composition Base Sequence Cloning, Molecular DNA Primers DNA, Bacterial Drug Design Electronic Data Processing Gene Expression Gene Expression Profiling Nucleic Acid Denaturation Nucleic Acid Hybridization Software Temperature Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Technical Report Journal Article

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J R Yoon, P D Laible, M Gu, H N Scott, F R Collart. 2002. Express primer tool for high-throughput gene cloning and expression.. https://doi.org/10.2144/02336bc03

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