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Double-stranded nef RNA interferes with human immunodeficiency virus type 1 replication.

Takuya Yamamoto·Shinya Omoto·Masaaki Mizuguchi·Hajime Mizukami·Harumi Okuyama·Noriko Okada·Nitin K Saksena·Ebiamadon A Brisibe·Kaori Otake·Yoichi R Fuji
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Abstract

RNA interference (RNAi) has been reported to be post-transcriptional gene silencing (PTGS) by approximately 500 nucleotide-(nt)-long double-stranded (ds) RNA that specifically targets homologous sequences of messenger RNA. In this report, we describe inhibition of HIV-1 transcription by synthetic dsRNAs constructed with mutated nef genes (nef dsRNAs) derived from long-term non-progressors (LTNPs) using cotransfection of the target gene-expressing plasmid and dsRNA. The effects of nef dsRNAs were examined with luciferase (Luc) reporter which is combined with the HIV-1 (SF2) LTR in persistently HIV-1-infected T cell and macrophage cell lines. At 48 hr, a defective nef dsRNA (556 nt) suppressed Luc activity more potently than did SF2 full-length nef dsRNA (744 nt), suggesting that approximately 500 nt-long nef dsRNA could interfere with the HIV-1 transcription.

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Gene Products, nefHIV Long-Term SurvivorsHIV-1HumansRNA InterferenceRNA, Double-StrandedVirus Replicationnef Gene Products, Human Immunodeficiency VirusJournal Article

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Takuya Yamamoto, Shinya Omoto, Masaaki Mizuguchi, Hajime Mizukami, Harumi Okuyama, Noriko Okada, Nitin K Saksena, Ebiamadon A Brisibe, Kaori Otake, Yoichi R Fuji. 2002. Double-stranded nef RNA interferes with human immunodeficiency virus type 1 replication.. https://doi.org/10.1111/j.1348-0421.2002.tb02768.x

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