Physical methods.

Alessandro Vitale

doi:10.1023/a:1021209702115

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PubMed · 12516856

Physical methods.

Alessandro Vitale

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Abstract

Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated.

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Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Cross-Linking Reagents Plant Proteins Plants Precipitin Tests Protein Binding Protein Interaction Mapping Recombinant Fusion Proteins Journal Article

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Alessandro Vitale. 2002. Physical methods.. https://doi.org/10.1023/a%3A1021209702115

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