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Multi-analyte analysis system using an antibody-based biochip.

Maria Cruz Moreno-Bondi·Jean Pierre Alarie·Tuan Vo-Dinh
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Abstract

A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip has a sampling platform of four-by-four microarrays of antibodies deposited onto a Nylon membrane substrate. The micro-arrayed antibodies can be interrogated simultaneously or sequentially using the biochip sensing array detector with the use of a diffractive optical element illuminating each antibody spot individually. The usefulness of the Ab biochip is illustrated by the measurements of immunoglobulin G (IgG) used as the model analyte system. The detection limit for Cy5-labeled IgG molecules was 13 pg.

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AnimalsAntibodies, MonoclonalAntigen-Antibody ComplexBiosensing TechniquesCalibrationEquipment DesignGoatsImmunoglobulin GMiceProtein Array AnalysisRabbitsSheepJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Maria Cruz Moreno-Bondi, Jean Pierre Alarie, Tuan Vo-Dinh. 2002-11-09. Multi-analyte analysis system using an antibody-based biochip.. https://doi.org/10.1007/s00216-002-1626-y

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