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PubMed · 12521301

Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray.

Eun Jung Chung·Young Kwan Sung·Mohammad Farooq·Younghee Kim·Sanguk Im·Won Young Tak·Yoon Jin Hwang·Yang Il Kim·Hyung Soo Han·Jung-Chul Kim·Moon Kyu Kim
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Abstract

We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

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Carcinoma, HepatocellularCluster AnalysisDNA PrimersDNA, ComplementaryDown-RegulationGene ExpressionGene Expression ProfilingGene Expression Regulation, NeoplasticHumansLiverLiver NeoplasmsNeoplasm ProteinsNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisRNA, MessengerReverse Transcriptase Polymerase Chain ReactionUp-RegulationComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Eun Jung Chung, Young Kwan Sung, Mohammad Farooq, Younghee Kim, Sanguk Im, Won Young Tak, Yoon Jin Hwang, Yang Il Kim, Hyung Soo Han, Jung-Chul Kim, Moon Kyu Kim. 2002-12-31. Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray.. https://pubmed.ncbi.nlm.nih.gov/12521301/

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