PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 12537367

Headspace solid-phase microextraction--comprehensive two-dimensional gas chromatography of wound induced plant volatile organic compound emissions.

Ranjini M M Perera·Philip J Marriott·Ian E Galbally
View original source ↗Export metadata ↓

Abstract

Plant emissions of volatile organic compounds from mechanically wounded Agrostis stolonifera, Pennisetum clandestinum, Eucalyptus leucoxylon and Trifolium repens have been sampled by headspace-solid phase microextraction (HS-SPME) and analysed by using comprehensive two-dimensional gas chromatography (GCxGC) for measurement of the plant emissions. GCxGC produces a fingerprint of the volatile organic compounds in a 2D separation space that may be approximately interpreted as a boiling point-polarity space, and may then be presented as a two-dimensional contour plot. This allows identification of sample-dependent variations in component distributions in the 2D plot, which will contain information about plant differences and should therefore facilitate recognition of different plant materials and displays the gross differences in volatiles between each plant species.

Explore related subjects

AgrostisChromatography, GasEucalyptusOils, VolatilePennisetumPlant OilsPlant Physiological PhenomenaTrifoliumJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Ranjini M M Perera, Philip J Marriott, Ian E Galbally. 2002. Headspace solid-phase microextraction--comprehensive two-dimensional gas chromatography of wound induced plant volatile organic compound emissions.. https://doi.org/10.1039/b208577a

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Mapping QTL for dollar spot resistance in creeping bentgrass (Agrostis stolonifera L.).

Dollar spot caused by Sclerotinia homoeocarpa F. T. Bennett is the most economically important turf disease on golf courses in North America. Dollar spot resistance in a creeping bentgrass cultivar would greatly reduce the frequency, costs, and environmental impacts of fungicide application. Little work has been done to understand the genetics of resistance to dollar spot in creeping bentgrass. Therefore, QTL analysis was used to determine the location, number and effects of genomic regions associated with dollar spot resistance in the field. To meet this objective, field inoculations using a single isolate were performed over 2 years and multiple locations using progeny of a full sib mapping population '549 x 372'. Dollar spot resistance seems to be inherited quantitatively and broad sense heritability for resistance was estimated to be 0.88. We have detected one QTL with large effect on linkage group 7.1 with LOD values ranging from 3.4 to 8.6 and explaining 14-36% of the phenotypic variance. Several smaller effect QTL specific to rating dates, locations and years were also detected. The association of the tightly linked markers with the LG 7.1 QTL based on 106 progeny was further examined by single marker analysis on all 697 progeny. The high significance of the QTL on LG 7.1 at a sample size of 697 (P < 0.0001), along with its consistency across locations, years and ratings dates, indicated that it was stable over environments. Markers tightly linked to the QTL can be utilized for marker-assisted selection in future bentgrass breeding programs.

Agrostis↗
PubMed2006

Engineered crops: transgenes go wild.

Genetically modified Agrostis stolonifera has escaped from cultivation. For the first time, a herbicide-resistant perennial weed has established itself in wild populations.

Agrostis↗
PubMed2006

Growth onset, senescence, and reproductive development of meadow species in mesocosms exposed to elevated O3 and CO2.

We studied the effects of elevated O3 (40-50 ppb) and CO2 (+100 ppm) alone and in combination on the growth onset, relative chlorophyll meter values, and reproductive development of meadow species grown in ground-planted mesocosms using open-top chambers. The 3-year study was conducted in the summers of 2002-2004. Elevated O3 decreased the early season coverage of plant communities and delayed the flowering of Campanula rotundifolia and Vicia cracca. The relative chlorophyll meter values of Fragaria vesca leaves were decreased by O3. Ozone also reduced the overall number of produced flowers, but as far as individual species were concerned, O3 had significant effects only on Campanula rotundifolia. In the case of Fragaria vesca, O3 decreased the fresh weight of individual berries. The effects of CO2 were less pronounced, and CO2 generally did not ameliorate the negative effects of O3. Changes in reproduction may affect the long-term fate of the whole community.

Agrostis↗