An efficient algorithm for minimal primer set selection.

Ming-Hua Hsieh; Wei-Che Hsu; Sung-Kay Chiu; Chi-Meng Tzeng

doi:10.1093/bioinformatics/19.2.285

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An efficient algorithm for minimal primer set selection.

Ming-Hua Hsieh·Wei-Che Hsu·Sung-Kay Chiu·Chi-Meng Tzeng

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Abstract

SUMMARY: We have developed U-PRIMER, a primer design program, to compute a minimal primer set (MPS) for any given set of DNA sequences. The U-PRIMER algorithm, which uses automatic variable fixing and automatic redundant constraint elimination to tackle the binary integer programming problem associated with the MPS selection problem. The program has been tested successfully with 32 adipocyte development-related genes and 9 TB-specific genes to obtain their respective MPSs. AVAILABILITY: A free copy of U-PRIMER implemented in C++ programming language is available from http://www.u-vision-biotech.com

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Adipocytes Algorithms Cell Differentiation DNA Primers Mycobacterium tuberculosis Oligonucleotide Array Sequence Analysis Quality Control Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Ming-Hua Hsieh, Wei-Che Hsu, Sung-Kay Chiu, Chi-Meng Tzeng. 2003-01-22. An efficient algorithm for minimal primer set selection.. https://doi.org/10.1093/bioinformatics%2F19.2.285

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