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Protein expression systems for structural genomics and proteomics.

Shigeyuki Yokoyama
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Abstract

One of the key steps of structural genomics and proteomics is high-throughput expression of many target proteins. Gene cloning, especially by ligation-independent cloning techniques, and recombinant protein expression using microbial hosts such as Escherichia coli and the yeast Pichia pastoris are well optimized and further robotized. Cell-free protein synthesis systems have been developed for large-scale production of protein samples for NMR (stable-isotope labeling) and X-ray crystallography (selenomethionine substitution). Protein folding is still a major bottleneck in protein expression. Cell-based and cell-free methods for screening of suitable samples for structure determination have been developed for achieving a high success rate.

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Cell-Free SystemCloning, MolecularGenesGenomicsProtein BiosynthesisProtein FoldingProteinsProteomicsJournal ArticleReview

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BibTeXRIS

Shigeyuki Yokoyama. 2003. Protein expression systems for structural genomics and proteomics.. https://doi.org/10.1016/s1367-5931(02)00019-4

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