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PubMed · 12569778

[Expression of hepatitis E virus structural gene in E. coli].

M Zhang·H Zhao·Y Jiang
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Abstract

OBJECTIVE: To obtain recombinant antigen for development of vaccine against hepatitis E virus. METHODS: Amplified the structural gene (5,816-7,126 nt) by PCR. The upstream primer was 5'-CCATATGAATTCAATAACCTC-3' and the downstream primer was 5'-GGGATCCTATAACTCCCGAGT-3'. Cut the PCR product with Nde I and BamHI, then inserted this fragment into the plasmid pET-11 where a cut was by the same restriction endonucleases. The expression plasmid named pEa47 was transformed into E. Coli BL21. The recombinant strains were grown at 37 degrees C and induced by IPTG. The recombinant protein was confirmed by Western blot analysis using serum from hepatitis E patient. RESULTS: The structural gene of hepatitis E virus from open reading frame 2,224-660 aa, was expressed in E. Coli BL21. Western blot assay showed that the expressed 50,000 recombinant protein specifically reacted with the serum antibody from the hepatitis E patient. CONCLUSION: The protein might be useful to develop vaccine against hepatitis E virus infection.

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Antigens, ViralCloning, MolecularEpitopesEscherichia coliGenes, ViralHepatitis E virusOpen Reading FramesRecombinant ProteinsViral Hepatitis VaccinesViral Structural ProteinsJournal Article

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M Zhang, H Zhao, Y Jiang. 1999-06-30. [Expression of hepatitis E virus structural gene in E. coli].. https://pubmed.ncbi.nlm.nih.gov/12569778/

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