A novel ColV plasmid encoding type IV pili.

Uri Gophna; Anat Parket; Joerg Hacker; Eliora Z Ron

doi:10.1099/mic.0.25858-0

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A novel ColV plasmid encoding type IV pili.

Uri Gophna·Anat Parket·Joerg Hacker·Eliora Z Ron

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Abstract

Many septicaemic Escherichia coli strains harbour ColV virulence plasmids. This paper describes pO78V, a conjugative ColV plasmid from an avian pathogenic E. coli strain that encodes type IV pili in addition to other virulence-related genes and tetracycline resistance. Plasmid location of type IV pili genes was demonstrated using Southern hybridization and expression of the pili was demonstrated using RT-PCR and phage sensitivity assays. This is a first report of a ColV plasmid encoding type IV pili. Plasmid pO78V is a mosaic plasmid containing replicons and other genes typical to both IncI1 and IncFII groups. As type IV pili of Gram-negative bacteria are involved in several stages of infection, their presence on a ColV virulence plasmid could expand the repertoire of pathogenesis-related genes.

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Animals Base Sequence Chickens Colicins Conjugation, Genetic Escherichia coli Escherichia coli Infections Fimbriae, Bacterial Molecular Sequence Data Plasmids Poultry Diseases Replicon Sepsis Virulence Virulence Factors Journal Article Research Support, Non-U.S. Gov't

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Uri Gophna, Anat Parket, Joerg Hacker, Eliora Z Ron. 2003. A novel ColV plasmid encoding type IV pili.. https://doi.org/10.1099/mic.0.25858-0

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