Hierarchical interactions control CD4 gene expression during thymocyte development.

Matthew Adlam; Gerald Siu

doi:10.1016/s1074-7613(03)00021-9

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Hierarchical interactions control CD4 gene expression during thymocyte development.

Matthew Adlam·Gerald Siu

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Abstract

CD4 gene regulation provides an ideal model for understanding the molecular events that drive T cell development. In this paper we use a transgenic approach to identify a CD4 LCR containing a stage-specific thymocyte enhancer (TE) and a region that protects against position effect variegation. Surprisingly, the TE acts indirectly through the previously defined proximal enhancer and is strongly induced upon commitment to the T cell lineage. We also describe a complex series of hierarchical control element interactions that orchestrate CD4 expression throughout thymopoiesis. These data provide a framework for understanding how CD4 gene expression is regulated in response to lineage commitment decisions.

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Animals CD4 Antigens CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Differentiation Enhancer Elements, Genetic Gene Expression Regulation, Developmental Locus Control Region Lymphopoiesis Mice Mice, Transgenic T-Lymphocyte Subsets Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Matthew Adlam, Gerald Siu. 2003. Hierarchical interactions control CD4 gene expression during thymocyte development.. https://doi.org/10.1016/s1074-7613(03)00021-9

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