Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools.

Ramit Mehr; Gitit Shahaf; Alex Sah; Michael Cancro

doi:10.1093/intimm/dxg025

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Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools.

Ramit Mehr·Gitit Shahaf·Alex Sah·Michael Cancro

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Abstract

B lymphopoiesis has historically been depicted as a unidirectional process, in which cohorts of developing cells transit through successive differentiative stages in an irreversible, synchronous manner. Here, we examine this view by combining kinetic analysis of developing B cell subsets in the bone marrow with mathematical modeling. Our bromo-deoxyuridine (BrdU) labeling data are incompatible with B cell development being a synchronous process, because labeling curves are non-linear. Moreover, we show that B cell development may not be completely unidirectional, because our results support the possibility of a phenotypic "reflux" among the immature to the pre-B cell subsets.

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Animals B-Lymphocytes Bone Marrow Bromodeoxyuridine Cell Differentiation Kinetics Mice Mice, Inbred BALB C Models, Biological Staining and Labeling Journal Article Research Support, Non-U.S. Gov't

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Ramit Mehr, Gitit Shahaf, Alex Sah, Michael Cancro. 2003. Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools.. https://doi.org/10.1093/intimm%2Fdxg025

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