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PubMed · 12621550

[Subcellular localization of basic Krüppel-like factor].

Xiao-Yun Ma·Mang-Ju Wang·Xiang-Hu Qu·Gui-Chun Xing·Yun-Ping Zhu·Fu-Chu He
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Abstract

To understand the function of basic Krüppel-like factor (BKLF), it was confirmed by direct fluorescence and indirect fluorescence observation that hBKLF was localized in nucleus, and distributed throughout nucleoplasm in a speckled pattern, except the nucleoli. This pattern is similar to many but not all transcription factors. To clarify the specific sequence responsible for its nuclear localization, a series of deletion mutants of GFP/hBKLF were constructed. By observing their subcellular localization, it was found that the three zinc fingers of hBKLF and the N-terminal aside from the fingers all served as nuclear localization signals (NLS); the sub-NLS of hBKLF was located in the N-terminus, including the CtBP-binding motif and the proline rich domain. These results provided a basis for further clarifying the function of BKLF.

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AnimalsCOS CellsCell NucleusDNA-Binding ProteinsGreen Fluorescent ProteinsHumansKruppel-Like Transcription FactorsLuminescent ProteinsMicroscopy, FluorescenceRecombinant Fusion ProteinsZinc FingersEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Xiao-Yun Ma, Mang-Ju Wang, Xiang-Hu Qu, Gui-Chun Xing, Yun-Ping Zhu, Fu-Chu He. 2003. [Subcellular localization of basic Krüppel-like factor].. https://pubmed.ncbi.nlm.nih.gov/12621550/

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