PubMed · 12622162
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
James E N Jonkman, Jim Swoger, Holger Kress, Alexander Rohrbach, Ernst H K Stelzer. 2003. Resolution in optical microscopy.. https://doi.org/10.1016/s0076-6879(03)60122-9
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
The techniques of single particle tracking (SPT) and optical force microscopy (OFM) as described above allow direct imaging of the motion of molecules in the membrane of live cells, and provide a means of controlling the movement by an almost noninvasive method. Combination of these techniques with other single-molecule methods, such as single-fluorophore imaging, allows direct comparison of motion at video rate (because faster than video rate imaging of fluorophore is still not generally feasible) to determine any effect due to the attached colloidal gold particle. Also, simultaneous use of the two techniques allows for monitoring two molecules, one at high time resolution. As such, the system can then be used in conjunction with green fluorescent protein (GFP) transfection to watch simultaneously the motion of an internal component of, say, a signaling pathway while seeing the motion of the transmembrane signaling receptor.
Explore the source record for details and available documents.
We have imaged a 2D buried Ni nanostructure at 8 nm resolution using coherent x-ray diffraction and the oversampling phasing method. By employing a 3D imaging reconstruction algorithm, for the first time we have experimentally determined the 3D structure of a noncrystalline nanostructured material at 50 nm resolution. The 2D and 3D imaging resolution is currently limited by the exposure time and the computing power, while the ultimate resolution is limited by the x-ray wavelengths. We believe these results pave the way for the development of atomic resolution 3D x-ray diffraction microscopy.