PCR-based methods for identification of Enterococcus species.

H Drahovská; D Kocíncová; M Seman; J Turna

doi:10.1007/bf02818665

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PCR-based methods for identification of Enterococcus species.

H Drahovská·D Kocíncová·M Seman·J Turna

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Abstract

Two DNA-based techniques were used for species identification of enterococci. PvuII digestion of the genus-specific PCR product yielded four different restriction profiles among 20 enterococcal species; one of them was species-specific for E. faecium. In the second case, 32 reference strains belonging to 20 enterococcal species were divided to 12 groups by amplification of internal transcribed spacer of rRNA operon. Interspecies and some intraspecies profile variability was determined. Both methods gave similar results.

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DNA, Intergenic DNA, Protozoan Deoxyribonucleases, Type II Site-Specific Enterococcus Polymerase Chain Reaction Species Specificity Journal Article Research Support, Non-U.S. Gov't

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H Drahovská, D Kocíncová, M Seman, J Turna. 2002. PCR-based methods for identification of Enterococcus species.. https://doi.org/10.1007/bf02818665

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