Protein analysis on a proteomic scale.

Eric Phizicky; Philippe I H Bastiaens; Heng Zhu; Michael Snyder; Stanley Fields

doi:10.1038/nature01512

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Protein analysis on a proteomic scale.

Eric Phizicky·Philippe I H Bastiaens·Heng Zhu·Michael Snyder·Stanley Fields

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Abstract

The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments.

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Animals Gene Expression Profiling Genome Humans Protein Array Analysis Protein Binding Proteome Proteomics Two-Hybrid System Techniques Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.Review

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Eric Phizicky, Philippe I H Bastiaens, Heng Zhu, Michael Snyder, Stanley Fields. 2003-03-13. Protein analysis on a proteomic scale.. https://doi.org/10.1038/nature01512

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