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PubMed · 12647002

Cryopreservation-induced decrease in heat-shock protein 90 in human spermatozoa and its mechanism.

Wen-Lei Cao·Yi-Xin Wang·Zu-Qiong Xiang·Zheng Li
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Abstract

AIM: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. METHODS: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. RESULTS: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1+/-3.2 and 243.0+/-21.6, respectively, while those after thawing were 23.2+/-2.5 and 105.7+/-28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. CONCLUSION: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.

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Blotting, WesternCryopreservationHSP90 Heat-Shock ProteinsHumansMaleSemen PreservationSperm MotilitySpermatozoaJournal Article

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Wen-Lei Cao, Yi-Xin Wang, Zu-Qiong Xiang, Zheng Li. 2003. Cryopreservation-induced decrease in heat-shock protein 90 in human spermatozoa and its mechanism.. https://pubmed.ncbi.nlm.nih.gov/12647002/

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