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PubMed · 12652786

[Recent advances in molecular diagnostic tests].

Hayato Miyachi
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Abstract

Recent advances in molecular biotechnologies have facilitated laboratory use of molecular diagnostic tests. Automated systems for amplification and detection, and lately for extraction, of nucleic acid sequences have been developed. The automated systems have allowed improvement of not only assay efficiency but also quality control of the tests. The information on the genome sequence from the human genome project has been studied to elucidate functions of genes and proteins and the clinical significance of nucleic acid sequences with post-genomics such as expression profiling using DNA microarray, proteomics, and single nucleotide polymorphism analysis, in conjunction with bioinformatics. Now the challenge is the development and application of these new technologies as clinical diagnostic tools. Design of a diagnostic array or panel must be developed with defined sequences based on interpretation of a huge quantity of experimental data to meet clinical demands. There is a need for development of generally available instruments that are inexpensive, practical and more importantly reproducible and reliable.

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Computational BiologyGene Expression ProfilingHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOligonucleotide Array Sequence AnalysisPolymorphism, Single NucleotideProteomicsReproducibility of ResultsSensitivity and SpecificityEnglish AbstractJournal ArticleReview

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Hayato Miyachi. 2002. [Recent advances in molecular diagnostic tests].. https://pubmed.ncbi.nlm.nih.gov/12652786/

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