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PubMed · 12653941

Amplifying whole insect genomes with multiple displacement amplification.

N Gorrochotegui-Escalante·W C Black
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Abstract

Many applications in insect genetics require repeated analyses on individual organisms. These include population genetic and genomics, linkage mapping, molecular systematics and map-based positional cloning. However, using the polymerase chain reaction, a limited number of analyses are possible with DNA isolated from whole bodies or parts of small or preserved specimens. We describe the optimization of a new technique, Multiple Displacement Amplification, for 100-400-fold amplification of whole mosquito genomes. We demonstrate that MDA amplifies genomic DNA directly from an adult leg or from organisms as small as a first instar mosquito larva. Genetic polymorphisms revealed at individual loci are the same whether using the original genomic DNA or MDA DNA as a template.

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AedesAnimalsDNAFemaleGenes, InsectGenetic TechniquesGenomePolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Single-Stranded ConformationalJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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N Gorrochotegui-Escalante, W C Black. 2003. Amplifying whole insect genomes with multiple displacement amplification.. https://doi.org/10.1046/j.1365-2583.2003.00401.x

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