PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 12661168

Multipurpose robot for automated cycle sequencing.

K Sawakami-Kobayashi·O Segawa·K Obata·E Hornes·M Yohda·H Tajima·M Machida
View original source ↗Export metadata ↓

Abstract

Paramagnetic beads have the superior advantages of easy separation and resuspension by controlling the magnetic filed. Previously, we have developed Magtration technology to automate paramagnetic bead handling and have built several automated instruments that handle 1-12 samples simultaneously. To achieve more high-throughput sample processing, two types of a 96-arrayed Integrated Magtration Unit (IMU) were developed, one installed with electromagnets and the other with thin rod-shaped magnets made of neodymium. A multipurpose robot (SX-96GC) equipped with the IMU was also developed for fully automatic processing of 96 samples in parallel. The cleanup of dye-terminator sequencing products was performed using the robot installed with the permanent magnet version of IMU. The results had quality comparable to those by the same protocol in manual handling or to those by the conventional protocols. The robot processed 96 samples in a microplate within 30 min. The protocol that can purify 384 samples within 1 h by processing two microplates concurrently was successfully designed.

Explore related subjects

Equipment DesignEquipment Failure AnalysisMagneticsMicrospheresPolymerase Chain ReactionRoboticsSequence Analysis, DNASpecimen HandlingEvaluation StudyJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

K Sawakami-Kobayashi, O Segawa, K Obata, E Hornes, M Yohda, H Tajima, M Machida. 2003. Multipurpose robot for automated cycle sequencing.. https://doi.org/10.2144/03343pf01

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2003

Microchip PCR.

Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable to surface chemistry of microchip components (inhibition of PCR), and the static and dynamic surface passivation strategies developed for the solution of these difficulties

Equipment Design↗
PubMed2003

Fluid leakage past tracheal tube cuffs: evaluation of the new Microcuff endotracheal tube.

OBJECTIVE: This study compared the recently introduced Microcuff endotracheal tube HVLP ICU featuring an ultrathin (7-microm) polyurethane cuff membrane with endotracheal tubes from different manufacturers regarding fluid leakage past the tube cuff. DESIGN: In vitro setup. MEASUREMENTS AND RESULTS: The following endotracheal tubes (ID 7.5 mm) were compared: Mallinckrodt HiLo, Microcuff HVLP ICU, Portex Profile Soft Seal, Rüsch Super Safety Clear, and Sheridan CF. A vertical PVC trachea model (ID 20 mm) was intubated, and cuffs were inflated to 10, 15, 20, 25, 30, and 60 cmH2O. Colored water (5 ml) was added to the top of the cuff. The amount of leaked fluid past the tube cuff within 5, 10, and 60 min was recorded. Experiments were performed four times using two examples of each tube brand. Fluid leakage past tube cuffs occurred in all conventional endotracheal tubes at cuff pressures from 10 to 60 cmH2O. In the Microcuff tube cuff pressure fluid leakage was observed within 10 min only at 10 cmH2O. Results with the Microcuff tube were significantly better than all other tube brands at cuff pressures of 10-30 cmH2O. CONCLUSIONS: Within the acceptable upper limit for tracheal cuff pressure (25-30 cmH2O) the Microcuff endotracheal tube was the only one of the tested tubes to prevent fluid leakage in our in vitro setup. In vivo studies are required to confirm these findings.

Equipment Design↗