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PubMed · 12665904

[Expression of full-length hepatitis E virus structural gene using baculovirus expression system].

Mingcheng Zhang·Yoo Yi·Chongbai Liu·Shengli Bi
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Abstract

OBJECTIVE: To obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein. METHODS: Full-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was picked and amplified and expression of HEV structural protein was tested by SDS-PAGE, Western blot and immunofluorescence methods. RESULTS: SDS-PAGE analysis showed HEV structural protein was highly expressed; Western blot and immunofluorescence assay showed that the expression product could specifically react with HEV positive sera, confirming the protein possessing HEV specific antigenicity. CONCLUSIONS: HEV structural protein was successfully expressed using baculovirus expression system.

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AnimalsBaculoviridaeCells, CulturedGene ExpressionGenesGenetic VectorsHepatitis E virusRecombinant ProteinsViral Structural ProteinsEnglish AbstractJournal Article

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Mingcheng Zhang, Yoo Yi, Chongbai Liu, Shengli Bi. 2002. [Expression of full-length hepatitis E virus structural gene using baculovirus expression system].. https://pubmed.ncbi.nlm.nih.gov/12665904/

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