Protein separations using colloidal magnetic nanoparticles.

Seyda Bucak; Deverraux A Jones; Paul E Laibinis; T Alan Hatton

doi:10.1021/bp0200853

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Protein separations using colloidal magnetic nanoparticles.

Seyda Bucak·Deverraux A Jones·Paul E Laibinis·T Alan Hatton

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Abstract

Phospholipid-coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein-laden particles are readily recovered from the feed solution using high-gradient magnetic filtration.

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Adsorption Chromatography, Ion Exchange Coated Materials, Biocompatible Colloids Cytochromes c Liposomes Magnetics Microspheres Nanotechnology Phospholipids Proteins Ultrafiltration Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Seyda Bucak, Deverraux A Jones, Paul E Laibinis, T Alan Hatton. Protein separations using colloidal magnetic nanoparticles.. https://doi.org/10.1021/bp0200853

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