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PubMed · 12680228

Is DD3 a new prostate-specific gene?

Orietta Gandini·Letizia Luci·Antonio Stigliano·Rossana Lucera·Franco Di Silverio·Vincenzo Toscano·Maria Rosaria Cardillo
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Abstract

The search for new and more specific molecular markers of prostatic cancers has led, in recent years, to the identification of the DD3 gene. Using reverse transcription (RT)-PCR, we investigated DD3 gene expression in human cell lines, in blood samples from healthy men and women donors and in various neoplastic and non-neoplastic tissues from the prostate and other organs. Whereas RT-PCR analysis using primers that amplified the region spanning exons 1 and 3 yielded DD3 gene expression in all samples examined, a primer downstream to exon 4 detected the expected size DD3 band only in benign and malignant prostatic tissues. These findings indicate that the only prostate specific region of DD3 is exon 4.

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Antigens, NeoplasmBiomarkers, TumorExonsFemaleGene Expression Regulation, NeoplasticHumansMaleOrgan SpecificityProstatic NeoplasmsRNA, MessengerReverse Transcriptase Polymerase Chain ReactionTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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Orietta Gandini, Letizia Luci, Antonio Stigliano, Rossana Lucera, Franco Di Silverio, Vincenzo Toscano, Maria Rosaria Cardillo. Is DD3 a new prostate-specific gene?. https://pubmed.ncbi.nlm.nih.gov/12680228/

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