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PubMed · 12683329

[Cell interaction with extracellular proteins during two-phase polymer system formation].

I V Voronkina·N V Kalmykova·Iu P Petrov·G P Pinaev·N V Tsupkina·O A Cherepanova
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Abstract

A modified method of investigation of surface properties of cells and proteins with the help of a two-phase polymer system dextran-500/polyethylenglycol-6000 was used. This method is based on changing the kinetics of two-phase system partitioning into phase on adding cells or macromolecules. These changes were registered by measuring the top phase optical density during the system partitioning at 500 nm. Cell lines L (NCTC clone 929), LS and A431, human keratinocytes and platelets, collagen I, laminin-1, laminin-2/4, and fibronectin were studied. The interaction between collagen and all cell types with the formation of complexes takes place during co-partitioning of cells and proteins in the two-phase system. Laminins differ in surface properties and in interaction with cells. Laminin-1 makes preferable complexes with cells of monolayer subline L (NCTC clone 929), but not with cells of suspension subline LS. No interaction of laminin-2/4 with L cells was detected, but, in contrast to laminin-1, this protein has the affinity to A431 cells. No interaction of L cells with fibronectin were detected.

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AnimalsCell AdhesionCell LineCollagenCountercurrent DistributionDextransErythrocytesExtracellular Matrix ProteinsFibronectinsHumansKeratinocytesLamininMiceMice, Inbred StrainsPolyethylene GlycolsJournal Article

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I V Voronkina, N V Kalmykova, Iu P Petrov, G P Pinaev, N V Tsupkina, O A Cherepanova. 2002. [Cell interaction with extracellular proteins during two-phase polymer system formation].. https://pubmed.ncbi.nlm.nih.gov/12683329/

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