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Enzymatic profiling system in a small-molecule microarray.

Qing Zhu·Mahesh Uttamchandani·Dongbo Li·Marie L Lesaicherre·Shao Q Yao
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Abstract

[reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.

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AnimalsCattleCoumarinsEnzymesFluorescent DyesProtein Array AnalysisSpectrometry, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov't

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Qing Zhu, Mahesh Uttamchandani, Dongbo Li, Marie L Lesaicherre, Shao Q Yao. 2003-04-17. Enzymatic profiling system in a small-molecule microarray.. https://doi.org/10.1021/ol034233h

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