A fluorogenic dye activated by the staudinger ligation.

George A Lemieux; Christopher L De Graffenried; Carolyn R Bertozzi

doi:10.1021/ja029013y

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A fluorogenic dye activated by the staudinger ligation.

George A Lemieux·Christopher L De Graffenried·Carolyn R Bertozzi

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Abstract

Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.

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Animals Azides Coumarins Fluorescence Fluorescent Dyes Kinetics Mice Phosphines Tetrahydrofolate Dehydrogenase Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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George A Lemieux, Christopher L De Graffenried, Carolyn R Bertozzi. 2003-04-23. A fluorogenic dye activated by the staudinger ligation.. https://doi.org/10.1021/ja029013y

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