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A versatile approach towards regioselective platinated DNA sequences.

Robert J Heetebrij·Martin de Kort·Nico J Meeuwenoord·Hans den Dulk·Gijs A van der Marel·Jacques H van Boom·Jan Reedijk
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Abstract

Undesired N(7) platination of 2'-deoxyguanosine residues at predetermined sites in an oligodeoxynucleotide (ODN) sequence is prevented by applying the sterically demanding diphenylcarbamoyl (DPC) as an O(6)-protecting group. The presence of a base-labile oxalyl linker between the immobilized 3'-nucleotide and controlled pore glass (CPG) allows cleavage of the protected ODN from the support and leaves DPC protection unaffected. This method provides an ODN with specifically blocked guanine-N(7) sites for platination. In the hexanucleotides prepared in this study, 5'-GGBGGT-3'(for B=T, C and A), a platinum GG adduct is introduced at G4,G5. These site-specific platinated hexamers were isolated in a yield of 65 %, and were fully characterized by using reversed-phase HPLC (high performance liquid chromotography), LCMS (liquid chromatography-mass spectrometry), MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry), PAGE (polyacrylamide gel electrophoresis) and Maxam-Gilbert sequencing analysis.

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Base SequenceCarbamatesChromatography, High Pressure LiquidCross-Linking ReagentsDNADNA AdductsElectrophoresis, Polyacrylamide GelGlassOligodeoxyribonucleotidesOrganoplatinum CompoundsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStereoisomerismJournal Article

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Robert J Heetebrij, Martin de Kort, Nico J Meeuwenoord, Hans den Dulk, Gijs A van der Marel, Jacques H van Boom, Jan Reedijk. 2003-04-14. A versatile approach towards regioselective platinated DNA sequences.. https://doi.org/10.1002/chem.200390209

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