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Correlation between E-test, disk diffusion, and microdilution methods for antifungal susceptibility testing of fluconazole and voriconazole.

Madonna J Matar·Luis Ostrosky-Zeichner·Victor L Paetznick·Jose R Rodriguez·Enuo Chen·John H Rex
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Abstract

The activities of fluconazole and voriconazole against isolates of Candida spp. (n = 400) were tested by the E-test, disk diffusion, and the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 broth microdilution-based reference methods. More than 96% of isolates found to be susceptible to fluconazole by the reference method were identified as susceptible by the agar-based methods. Lesser degrees of correlation with the reference method were seen for isolates identified as resistant by the agar-based methods. Interpretive categories are not available for voriconazole, but results qualitatively similar to those for fluconazole were seen. The agar-based E-test and disk diffusion methods are reliable alternatives to the NCCLS M27-A2 reference microdilution method for isolates that test susceptible to fluconazole.

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Antifungal AgentsCandidaDiffusionFluconazoleMicrobial Sensitivity TestsPyrimidinesTriazolesVoriconazoleComparative StudyJournal Article

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Madonna J Matar, Luis Ostrosky-Zeichner, Victor L Paetznick, Jose R Rodriguez, Enuo Chen, John H Rex. 2003. Correlation between E-test, disk diffusion, and microdilution methods for antifungal susceptibility testing of fluconazole and voriconazole.. https://doi.org/10.1128/aac.47.5.1647-1651.2003

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