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Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization.

Salah D M Hasan·Walderez Gambale·Ricardo L Zollner·Maria H A Santana
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Abstract

Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays.

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AllergensBiomassConfidence IntervalsFermentationHelminthosporiumHydrogen-Ion ConcentrationKineticsJournal Article

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Salah D M Hasan, Walderez Gambale, Ricardo L Zollner, Maria H A Santana. 2003. Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization.. https://doi.org/10.1385/abab%3A106%3A1-3%3A403

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