[Molecular construction (superstructures) with adjustable properties based on double-stranded nucleic acids].

Iu M Evdokimov; V I Salianov; Iu D Nechipurenko; S G Skuridin; M A Zakharov; F Spener; M Palumbo

PubMed · 12723480

[Molecular construction (superstructures) with adjustable properties based on double-stranded nucleic acids].

Iu M Evdokimov·V I Salianov·Iu D Nechipurenko·S G Skuridin·M A Zakharov·F Spener·M Palumbo

Abstract

Formation of molecular construction that consists of double-stranded molecules of nucleic acids (or synthetic polynucleotides) located at the distance 30-50 A in the spatial structure of particles of their cholesteric liquidcrystalline dispersion and crosslinked by polymeric chelate bridges is described. The resulting superstructure, which possesses peculiar physicochemical properties, could be used as integral biosensor whose properties depend on temperature, the presence of chemical or biologically active compounds of different nature, etc.

Explore related subjects

Antibiotics, Antineoplastic Biochemistry Chemistry, Physical DNA Models, Molecular Nucleic Acid Conformation Nucleic Acids Optics and Photonics English Abstract Journal Article Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Iu M Evdokimov, V I Salianov, Iu D Nechipurenko, S G Skuridin, M A Zakharov, F Spener, M Palumbo. [Molecular construction (superstructures) with adjustable properties based on double-stranded nucleic acids].. https://pubmed.ncbi.nlm.nih.gov/12723480/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2003

Immediate effects of anticancer drugs on mitochondrial oxygen consumption.

The evolving role of mitochondria as a target for many anticancer drugs (e.g. platinum-based compounds, alkylating agents and anthracyclines) prompted us to investigate their immediate effects on the mitochondrial respiratory chain. For this purpose, we used a phosphorescence analyzer that measures [O(2)] in solution. The [O(2)] of solutions containing an appropriate substrate and various cell lines, tumors from patients or beef heart submitochondrial particles (SMPs) declined almost linearly (r>0.99) as a function of time, indicating that the kinetics of cellular oxygen consumption were zero order. Rotenone inhibited respiration, confirming that oxygen was consumed by the respiratory chain. Exposure to a clinically relevant concentration of cisplatin (5 microM at 37 degrees for 1-3 hr) had no effect on the respiration in cells or in SMP. Higher cisplatin concentrations (10-99 microM at 37 degrees for 1-3 hr) produced <25% inhibition. Incubations with 4-hydroperoxycyclophosphamide (50-100 microM at 37 degrees for 1 hr) inhibited oxygen consumption in SMP ( approximately 70% inhibition at 50 microM) and in cells ( approximately 30% inhibition at 50 microM). Incubations (37 degrees for 1 hr) of SMP with doxorubicin (25-100 microM) and daunorubicin (25-100 microM) had no inhibitory effect on the respiration. By contrast, incubations (37 degrees for 1 hr) of cells with doxorubicin (5-20 microM) and daunorubicin (2-20 microM) produced significant inhibition. We conclude that cisplatin does not directly damage the energy converting mechanism of mitochondria. On the other hand, comparable exposures to alkylating agents and anthracyclines produce immediate and dose-dependent impairment of cellular respiration.

Antibiotics, Antineoplastic↗

PubMed2003

Treatment for asymptomatic anthracycline-induced cardiac dysfunction in childhood cancer survivors: the need for evidence.

Explore the source record for details and available documents.

Antibiotics, Antineoplastic↗

PubMed2003

Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy.

Thermally responsive elastin-like polypeptides (ELPs) were synthesized by recombinant DNA techniques and conjugated to doxorubicin through an acid-labile hydrazone bond to enable release of the drug in the acidic environment of lysosomes. The thermal properties, intracellular localization and cytotoxicity of the conjugate were investigated in this study. The conjugation procedure resulted in a mixed population of free ELP and ELP-doxorubicin (ELP-dox) conjugates that exhibit a broader transition than the parent ELP. A simple centrifugation procedure was developed to purify the ELP-dox conjugate from other reactants and resulted in a sharper thermal transition, similar to the parent ELP. The ELP was endocytosed by squamous cell carcinoma cells (FaDu) and trafficked into lysosomes, as observed by the colocalization of the ELP with a lysosome-specific dye through confocal fluorescence microscopy. Interestingly, both the ELP-dox conjugate and free drug exhibited near equivalent in vitro cytotoxicity, although their subcellular localization was significantly different. The free drug was largely concentrated in the nucleus, while the conjugate was dispersed throughout the cytoplasm with limited nuclear accumulation. These differences are significant because they suggest a different mechanism of cytotoxicity for the conjugate as compared with the free drug.

Antibiotics, Antineoplastic↗