Human B-blast specific target determinants in CML: a methodological study.

H E Johnsen

doi:10.1111/j.1399-0039.1980.tb00903.x

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Human B-blast specific target determinants in CML: a methodological study.

H E Johnsen

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Abstract

A method for preparation of purified human PWM stimulated B lymphoblasts and their usefulness as targets in CML is described. The purified B lymphoblasts were found to carry surface membrane immunoglobulin (SmIg) and HLA-DR antigens and were non E-rosette forming. In contrast, PHA-stimulated lymphoblasts were found to be E-rosetting, SmIg negative and without serologically detectable HLA-DR antigens, and thus were characterized as T lymphoblasts. Using B and T lymphoblasts in parallel as targets in CML, it was possible to demonstrate target determinants exclusively expressed by the B lymphoblasts.

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B-Lymphocytes Biomarkers Cell Separation HLA-DR Antigens Humans Immunity, Cellular Phytohemagglutinins T-Lymphocytes T-Lymphocytes, Cytotoxic Journal Article Research Support, Non-U.S. Gov't

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H E Johnsen. 1980. Human B-blast specific target determinants in CML: a methodological study.. https://doi.org/10.1111/j.1399-0039.1980.tb00903.x

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