Genetic immunization is augmented by murine polyomavirus VP1 pseudocapsids.

E Rollman; T Ramqvist; B Zuber; K Tegerstedt; A Kjerrström Zuber; J Klingström; L Eriksson; K Ljungberg; J Hinkula; B Wahren; T Dalianis

doi:10.1016/s0264-410x(03)00049-5

PubMed · 12744856

Genetic immunization is augmented by murine polyomavirus VP1 pseudocapsids.

E Rollman·T Ramqvist·B Zuber·K Tegerstedt·A Kjerrström Zuber·J Klingström·L Eriksson·K Ljungberg·J Hinkula·B Wahren·T Dalianis

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Abstract

To improve immune responses induced by DNA immunization, murine polyomavirus major capsid protein (VP1) pseudocapsids were complexed with a DNA plasmid encoding the p37 (p24 and p17) nucleocapsid proteins of the human immunodeficiency virus type 1 (HIV-1). A 10-fold increase in antibody titer was noted in mice given DNA plasmid together with VP1 pseudocapsids in comparison to animals that received DNA plasmid alone. Cell mediated responses to HIV-1 p24 occurred, but were not significantly augmented by delivering the DNA as a VP1 complex. We have consequently for the first time shown a carrier/adjuvant effect of polyomavirus pseudocapsids that strongly increased the humoral immune response in DNA immunization.

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Animals Antibodies, Viral Antibody Formation Capsid Proteins Cloning, Molecular Mice Mice, Inbred C57BL Polyomavirus Polyomavirus Infections Vaccines, DNA Viral Vaccines Journal Article Research Support, Non-U.S. Gov't

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E Rollman, T Ramqvist, B Zuber, K Tegerstedt, A Kjerrström Zuber, J Klingström, L Eriksson, K Ljungberg, J Hinkula, B Wahren, T Dalianis. 2003-06-02. Genetic immunization is augmented by murine polyomavirus VP1 pseudocapsids.. https://doi.org/10.1016/s0264-410x(03)00049-5

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