Modeling tissue-specific signaling and organ function in three dimensions.

Karen L Schmeichel; Mina J Bissell

doi:10.1242/jcs.00503

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Modeling tissue-specific signaling and organ function in three dimensions.

Karen L Schmeichel·Mina J Bissell

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Abstract

In order to translate the findings from basic cellular research into clinical applications, cell-based models need to recapitulate both the 3D organization and multicellular complexity of an organ but at the same time accommodate systematic experimental intervention. Here we describe a hierarchy of tractable 3D models that range in complexity from organotypic 3D cultures (both monotypic and multicellular) to animal-based recombinations in vivo. Implementation of these physiologically relevant models, illustrated here in the context of human epithelial tissues, has enabled the study of intrinsic cell regulation pathways and also has provided compelling evidence for the role of the stromal compartment in directing epithelial cell function and dysfunction. Furthermore the experimental accessibility afforded by these tissue-specific 3D models has implications for the design and development of cancer therapies.

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Animals Breast Neoplasms Carcinoma Epithelial Cells Humans Mice Models, Animal Models, Biological Organ Culture Techniques Organ Specificity Signal Transduction Stromal Cells Viscera Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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Karen L Schmeichel, Mina J Bissell. 2003-06-15. Modeling tissue-specific signaling and organ function in three dimensions.. https://doi.org/10.1242/jcs.00503

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