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In vitro bone formation using muscle-derived cells: a new paradigm for bone tissue engineering using polymer-bone morphogenetic protein matrices.

Helen H Lu·Michelle D Kofron·Saadiq F El-Amin·Mohammed A Attawia·Cato T Laurencin
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Abstract

Over 800,000 bone grafting procedures are performed in the United States annually, creating a demand for viable alternatives to autogenous bone, the grafting standard in osseous repair. The objective of this study was to examine the efficacy of a BMP-polymer matrix in inducing the expression of the osteoblastic phenotype and in vitro bone formation by muscle-derived cells. Specifically, we evaluated the ability of bone morphogenetic protein-7 (BMP-7), delivered from a poly(lactide-co-glycolide) (PLAGA) matrix, to induce the differentiation of cells derived from rabbit skeletal muscle into osteoblast-like cells and subsequently form mineralized tissue. Results confirmed that muscle-derived cells attached and proliferated on the PLAGA substrates. BMP-7 released from PLAGA induced the muscle-derived cells to increase bone marker expression and form mineralized cultures. These results demonstrate the efficacy of a BMP-polymer matrix in inducing the expression of the osteoblastic phenotype by muscle-derived cells and present a new paradigm for bone tissue engineering.

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Absorbable ImplantsAnimalsBone Morphogenetic Protein 7Bone Morphogenetic ProteinsCalcification, PhysiologicCell AdhesionCell DifferentiationCell DivisionLactic AcidMuscle, SkeletalOsteoblastsOsteogenesisPhenotypePolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersRabbitsTissue EngineeringTransforming Growth Factor betaJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Helen H Lu, Michelle D Kofron, Saadiq F El-Amin, Mohammed A Attawia, Cato T Laurencin. 2003-06-13. In vitro bone formation using muscle-derived cells: a new paradigm for bone tissue engineering using polymer-bone morphogenetic protein matrices.. https://doi.org/10.1016/s0006-291x(03)00858-1

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