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Tissue macroarray: a simple and cost-effective method for high-throughput studies.

Liang Wang·Michael T Deavers·Anais Malpica·Elvio G Silva·Jinsong Liu
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Abstract

The tissue microarray technique is a powerful technique that can examine many clinical specimens in a single slide. However, this technique requires a specialized instrument for the construction of tissue microarray block. The authors describe an alternative tissue macroarray technique for the simultaneous analysis of multiple clinical specimens on a single slide. As an example of this method, the authors assembled 12 tumor samples from 12 different paraffin blocks on a glass slide and then immunostained with hMLH1 antibody. This simple method does not need specialized equipment and should be an alternative efficient approach for large-scale clinical specimens.

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Antibodies, MonoclonalFemaleHumansImmunohistochemistryMicrotomyOvarian NeoplasmsParaffin EmbeddingStaining and LabelingJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Liang Wang, Michael T Deavers, Anais Malpica, Elvio G Silva, Jinsong Liu. 2003. Tissue macroarray: a simple and cost-effective method for high-throughput studies.. https://doi.org/10.1097/00129039-200306000-00015

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