Molluscan shell matrix characterization by preparative SDS-PAGE.

Frédéric Marin

doi:10.1100/tsw.2003.30

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Molluscan shell matrix characterization by preparative SDS-PAGE.

Frédéric Marin

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Abstract

The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated "blind" on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.

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Animals Antibodies Chemical Fractionation Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Freund's Adjuvant Glycoproteins Immunization Mollusca Protein Denaturation Rabbits Sodium Dodecyl Sulfate Journal Article Research Support, Non-U.S. Gov't

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Frédéric Marin. 2003-05-05. Molluscan shell matrix characterization by preparative SDS-PAGE.. https://doi.org/10.1100/tsw.2003.30

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