Separation and identification of different refolding components.

Ming Li; Zhiguo Su

doi:10.1016/s0168-1656(03)00108-1

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Separation and identification of different refolding components.

Ming Li·Zhiguo Su

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Abstract

A dual-gradient ion-exchange chromatography (IEC) refolding process has been successfully applied to renature lysozyme at high concentration. The different refolding components were separated by size-exclusion chromatography with low concentrations of urea and NaCl added into elution buffer. The hydrodynamic characteristics during separation and the hydrophobic properties of these components were also tested.

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Chromatography, Ion Exchange Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Muramidase Protein Folding Sodium Chloride Urea Journal Article Research Support, Non-U.S. Gov't

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Ming Li, Zhiguo Su. 2003-06-26. Separation and identification of different refolding components.. https://doi.org/10.1016/s0168-1656(03)00108-1

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