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PubMed · 1281647

Rescuing transgene expression by co-integration.

A J Clark·A Cowper·R Wallace·G Wright·J P Simons
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Abstract

To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep beta-lactoglobulin gene with two poorly expressed beta-lactoglobulin-derived hybrid genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. "Rescuing" transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.

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AnimalsDNAGene Expression RegulationGenetic EngineeringLactoglobulinsMiceMice, TransgenicMilk ProteinsRNAJournal Article

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A J Clark, A Cowper, R Wallace, G Wright, J P Simons. 1992. Rescuing transgene expression by co-integration.. https://doi.org/10.1038/nbt1192-1450

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