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Functional genomics guided with MR imaging: mouse tumor model study.

Samira Guccione·Yi-Shan Yang·Gongyi Shi·Daniel Y Lee·King C P Li·Mark D Bednarski
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Abstract

To gain a better understanding of gene expression patterns in tumors, the authors used contrast material-enhanced magnetic resonance (MR) imaging to noninvasively characterize regions within the same tumor to provide a correlate for genomic analysis. Gene expression profiles of samples from a mouse tumor model obtained from contrast-enhanced and nonenhanced regions within the same tumor were compared with MR imaging and functional genomics. From these samples, 11000 genes were analyzed: 10 genes were up-regulated in the contrast-enhanced areas, and one gene was up-regulated in the nonenhanced regions. Several of these genes encode extracellular matrix proteins. Findings in this study demonstrate that MR imaging can serve as a powerful noninvasive tool for characterizing different regions of tumors to guide genomic analysis with high spatial and temporal resolution.

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AnimalsBlotting, WesternCarcinoma, Squamous CellContrast MediaDisease ProgressionGadolinium DTPAGene Expression ProfilingGenomicsMagnetic Resonance ImagingMiceOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Samira Guccione, Yi-Shan Yang, Gongyi Shi, Daniel Y Lee, King C P Li, Mark D Bednarski. 2003-06-23. Functional genomics guided with MR imaging: mouse tumor model study.. https://doi.org/10.1148/radiol.2282020907

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