p53-induced gene expression analysis.

Joanna K Sax; Wafik S El-Deiry

doi:10.1385/1-59259-408-5:65

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p53-induced gene expression analysis.

Joanna K Sax·Wafik S El-Deiry

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Abstract

Researchers in the p53 field have successfully used many high-throughput screening technologies to analyze and characterize p53-induced gene expression. This chapter will focus on one such technology, the Affymetrix GeneChip. DNA-Chip technology has grown rapidly over the last several years. The ability to hybridize RNA from a sample to thousands to tens of thousands of known and unknown cDNAs spotted on a microarray chip has led to the explosion of information ranging from macro-global expression pattern changes to micro-gene-specific expression changes. The relative ease of making a nonradioactive probe from either total RNA or mRNA to be hybridized to a GeneChip makes microarray technology highly attractive.

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Animals DNA, Complementary Gene Expression Profiling Gene Expression Regulation Oligonucleotide Array Sequence Analysis RNA Tumor Suppressor Protein p53 Journal Article

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Joanna K Sax, Wafik S El-Deiry. 2003. p53-induced gene expression analysis.. https://doi.org/10.1385/1-59259-408-5%3A65

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