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Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8.

Julia M Pettinari·Luciano Chaneton·Gustavo Vazquez·Alexander Steinbüchel·Beatriz S Méndez
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Abstract

The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented.

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3-Hydroxybutyric AcidAzotobacter vinelandiiDNA Transposable ElementsEscherichia coliGene Expression Regulation, BacterialGenes, BacterialMultigene FamilyNitrogen FixationRecombination, GeneticSequence Analysis, DNAJournal ArticleResearch Support, Non-U.S. Gov't

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Julia M Pettinari, Luciano Chaneton, Gustavo Vazquez, Alexander Steinbüchel, Beatriz S Méndez. 2003. Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8.. https://doi.org/10.1016/s0147-619x(03)00009-x

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