Analysis of complement-bound hepatitis B virus complexes by an immuno-capture polymerase chain reaction method.

S Y Wang; R Liu; J Y Zhang; Q Z Lian; X X Peng

doi:10.1046/j.1365-3083.2003.01282.x

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Analysis of complement-bound hepatitis B virus complexes by an immuno-capture polymerase chain reaction method.

S Y Wang·R Liu·J Y Zhang·Q Z Lian·X X Peng

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Abstract

Hepatitis B Virus (HBV) can be present in the circulating blood either as a free virus or as a virion-immunoglobulin (Ig) complex with or without complement. However, information regarding the complement-bound HBV circulating immune complexes (CIC) in HBV-infected patients is currently not available. In the present study, we have combined immuno-capture and polymerase chain reaction (PCR) and developed a quick method of high specificity for the detection of complement-bound HBV CIC. We found that the frequency of HBV-factor B was associated with clinical types of hepatitis B (HB) but not with that of HBV-C1q. Moreover, increased frequency at P < 0.05 were found for HBV/C1q-CIC in the group with normal total bilirubin (TBIL) and for HBV/factor B-CIC in the group with positive hepatitis B e antigen (HBeAg). These findings suggest that the immuno-capture PCR (iPCR) for the detection of HBV-bound CIC is a valuable method for analysis of the composition of the immune complexes and for the understanding of host immune response and immune pathogenesis in HBV-infected individuals. In summary, iPCR is a valuable method for analysis of the composition of the immune complexes, which may provide new and valuable insights into HBV pathogenesis.

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Adolescent Adult Antigen-Antibody Complex Complement C1q Complement Factor B Complement System Proteins DNA, Viral Female Hepatitis B virus Humans Male Middle Aged Polymerase Chain Reaction Journal Article Research Support, Non-U.S. Gov't

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S Y Wang, R Liu, J Y Zhang, Q Z Lian, X X Peng. 2003. Analysis of complement-bound hepatitis B virus complexes by an immuno-capture polymerase chain reaction method.. https://doi.org/10.1046/j.1365-3083.2003.01282.x

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