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PubMed · 1283747

DP epitope mapping by using T-cell clones.

A Urlacher·A Dormoy·M M Tongio
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Abstract

To determine whether a correlation exists between the genomic HLA class II DP DNA polymorphism and cell surface expression and to detect the DP epitopes responsible for alloreactivity, anti-DP T-cell clones were generated against new PLT blank RFLP DPa and DPb-defined specificities. The clones were tested on the 10th IHWS B-LCLs and on local panel cells. Oligotyping of the tested cells made it possible to (a) correlate the DPa specificity with the DPB1*0402 specificity and (b) split DPb into DPB1*1001 and DPB1*1401. By comparing DNA sequences of the second exon to panel reactivity, the epitopes responsible for DPB1*1001 and 1401 were defined and attributed to beta-chain residues contributing to peptide selection inside the HLA groove. However, DNA sequences could not explain anti-DPa allospecificity, indicating that another structure not yet definable may be involved.

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Amino Acid SequenceAntibodies, MonoclonalB-LymphocytesClone CellsEpitopesHLA-DP AntigensHumansLymphocyte Culture Test, MixedMolecular Sequence DataPeptide MappingPolymorphism, GeneticPolymorphism, Restriction Fragment LengthSequence Homology, Amino AcidT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov't

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A Urlacher, A Dormoy, M M Tongio. 1992. DP epitope mapping by using T-cell clones.. https://doi.org/10.1016/0198-8859(92)90017-h

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