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PubMed · 12855808

A B cell-based sensor for rapid identification of pathogens.

Todd H Rider·Martha S Petrovick·Frances E Nargi·James D Harper·Eric D Schwoebel·Richard H Mathews·David J Blanchard·Laura T Bortolin·Albert M Young·Jianzhu Chen·Mark A Hollis
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Abstract

We report the use of genetically engineered cells in a pathogen identification sensor. This sensor uses B lymphocytes that have been engineered to emit light within seconds of exposure to specific bacteria and viruses. We demonstrated rapid screening of relevant samples and identification of a variety of pathogens at very low levels. Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications.

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AequorinAntibodies, BacterialAntibodies, ViralB-LymphocytesBacillus anthracisBacteriaBacteriological TechniquesBiosensing TechniquesCell LineColony Count, MicrobialEncephalitis Virus, Venezuelan EquineEscherichia coli O157Foot-and-Mouth Disease VirusImmunoglobulin Variable RegionLightReceptors, Antigen, B-CellSensitivity and SpecificityTime FactorsTransfectionVirusesYersinia pestisJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Todd H Rider, Martha S Petrovick, Frances E Nargi, James D Harper, Eric D Schwoebel, Richard H Mathews, David J Blanchard, Laura T Bortolin, Albert M Young, Jianzhu Chen, Mark A Hollis. 2003-07-11. A B cell-based sensor for rapid identification of pathogens.. https://doi.org/10.1126/science.1084920

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